Cacao Lab


What actually are antioxidants?

Bean to bar dark chocolates are great for health because they tend to contain less sugar and are rich in antioxidants. However, what actually are antioxidants? Here’s a little infographic for you to better understand the importance of antioxidants in our everyday life!


Antioxidant Comparisons

At Benns, we believe in transparency and honesty, and wanted to make sure our bean to bar chocolates were actually as healthy as we believed them to be. What better way to do this than to put them to the test against commercial chocolates. As they say, the proof is in the (chocolate) pudding.

(1) 72% Dark Bean to Bar vs. 72% Commercial Chocolate
We carried out an antioxidant analysis comparing the 72% dark chocolate we bought from the market (commercial chocolates) and the 72% dark bean to bar chocolate we made using cacao beans from Sungai Ruan and Vung Tau estates.

From the results (shown in table 1.0) and consistent with other independent research, we were pleased our proposition empirically supported that bean to bar chocolates do contain much higher antioxidant properties than commercial dark chocolate, even if they of the same cocoa content. This is likely because in producing bean to bar chocolates, no artificial additives are used and no dutching processes (which reduce antioxidant count) are used, allowing the chocolate to retain more natural antioxidants of the cacao bean!




2) Cacao Nibs vs Berries
The second antioxidant comparison we did was between cacao nibs and berries.
Based on research done on the total phenolic content of common fruits and vegetables, we identified berries to contain one of the highest amounts of antioxidants. (refer to Figure 1.0 below )
 

Benns Chocolate carried out antioxidant testing for cacao nibs of three different origins (Malaysia, Vietnam, and Venezuela). The results show that Malaysian Cacao Nibs have up to 4 times higher the total phenolic content in some berries! Meanwhile, the Venezuela and Vietnam Cacao Nibs have a slightly lower total phenolic content which is still far above the phenolic antioxidant content of most fruits.


References:
Wang, H.; Cao, G.; Prior, R. L. Total antioxidant capacity of fruits. J. Agric. Food Chem. 1996, 44, 701-705.
Macheix, J.-J.; Fleuriet, A.; Billot, J. Fruit Phenolics; CRC Press: Boca Raton, FL, 1990.
Heinonen, I. M.; Meyer, A. S.; Frankel, E. N. Antioxidant activity of berry phenolics on human low-density lipoprotein and liposome oxidation. J. Agric. Food Chem. 1998, 46, 4107-4112.
Wang, S. Y.; Jiao, H. Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen. J. Agric. Food Chem. 2000, 48, 5672-5676.
Prior, R. L.; Cao, G.; Martin, A.; Sofic, E.; McEwen, J.; O’Brien, C.; Lischner, N.; Ehlenfeldt, M.; Kalt, W.; Krewer, G.; Mainland, C. M. Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity, and variety of Vaccinium species. J. Agric. Food Chem. 1998, 46, 2686-2693.
Wang, H.; Cao, G.; Prior, R. L. Total antioxidant capacity of fruits. J. Agric. Food Chem. 1997, 44, 701-705. (10) Constantino, L.; Albasino, A.; Rastelli, G.; Benvenuti, S. Activity of polyphenolic crude extracts as scavengers of superoxide radicals and inhibitors of xanthine oxidase. Planta Med. 1992, 58, 342-344.
Marja P. Ka¨hko¨nen, Anu I. Hopia, and Marina Heinonen. Berry Phenolics and Their Antioxidant Acitivity. J.Agric. Food Chem. 2001, 49, 4076-4082.

 

(3) Cacao Tea vs. Berries, Coffee and Teas

Our third comparison was that of cacao tea with berries, coffees and teas.

 

Berries, Coffee and Teas

Based on research conducted by Marja P. Ka¨hko¨nen et al., in 2001, bilberry contains the highest antioxidant content followed by raspberry, strawberry, cowberry, and apple (see table 2)

 

Meanwhile, we conducted a study together with UCSI University and found that the total phenolic content of black tea and coffee were 3415.6 mg GAE/100 and 2459.7mg GAE/100g respectively.

 

After testing Benn’s Cacao Teas, we found that for Benn’s Vietnam Single Estate Cacao Tea, the TPC was highest yet at 3793.5mg GAE/100g! Meanwhile, Benns’ Malaysia Single Estate Cacao Tea had a TPC of 3366.0 mg GAE/100g. For a summary of the results and comparisons, refer to the table below.

 

References:
Marja P. Ka¨hko¨nen, Anu I. Hopia, and Marina Heinonen, 2001, Berry Phenolics and Their Antioxidant Activity, J. Agric. Food Chem. 49, 4076-4082
 

(4) Antioxidant activity and Antioxidant properties

 

Our fourth comparison was that of cacao tea, coffee, green tea and black tea on Theobromine and caffeine content. 

 

What is Theobromine? It’s a compound that exists naturally in a variety of plants, most notably the cacao bean. (Other natural sources of theobromine include coffee beans, guarana berries, and some tea leaves.)

 

It was found that the second highest theobromine (140.22µg/mL)(Figure 1.0)  was in Benns Malaysia cacao tea but low in caffeine content (5.96mg/L) (Figure 1.1) as compared to all cacao tea origins!

 

Baggott et al. (2013) reported that appropriate intake of theobromine may contribute to the positive effects of chocolate, however at higher intake, the effect may become negative.

 

In view of the theobromine studies carried out by other researchers, the concentration of theobromine in cacao teas in this study was considered relatively low (120.14 to 164.98µg/mL) and safe to consume as this cacao tea was made from natural product (cacao beans).

 

 

(5) Sensory Evaluation

 

A Quantitative Descriptive Analysis (QDA) was conducted to evaluate the sensory attributes for cacao tea (Vietnam, Malaysia, and Venezuela), green tea, black tea and coffee. Twelve trained panellists were chosen to evaluate eight sensory attributes including sweetness, sourness, bitterness, astringency, chocolatey, grassiness, toasty and aromatic on a 15-cm line scale.

 

 

As shown in Table 1.1, Benns Vietnam cacao tea possessed the highest sweetness as compared to Malaysia and Venezuela cacao tea. Green tea, black tea, and coffee indicated a lower score as compared to cacao tea.

 

Benns Malaysia origin cacao tea perceived the strongest chocolatey profile (3.75), followed Venezuela origin cacao tea (2.88), and Vietnam origin cacao tea (2.28).

 

(6) Hedonic Survey

 

Hedonic survey was assessed to determine the degree of consumer acceptability of cacao tea (Vietnam, Malaysia, Venezuela) with comparison to green tea, black tea and coffee. 105 untrained respondents were requested to rate their liking using a 9-point hedonic scale. 

 

According to Table 1.2, Benns Vietnam origin cacao tea was the most acceptable sample with a mean hedonic score of 6.26! Based on the comments from respondents, Benns Vietnam origin cacao tea and Malaysia origin cacao tea had a nicer aroma compared to green tea, black tea and coffee.

 

 

 

References
Abhay, S.M., Hii, C.L., Law, C.L., Suzannah, S. and Djaeni, M., 2016. Effect of hot-air drying  temperature on the polyphenol content and the sensory properties of cocoa beans. International Food Research Journal, 23(4), 1479-1484Aprotosoaie, A.C., Luca, S.V. and Miron, A., 2016. Flavor chemistry of cocoa and cocoa products—an overview. Comprehensive Reviews in Food Science and Food Safety, 15(1), 73-91.Bell, L.N., Wetzel, C.R. and Grand, A.N., 1996. Caffeine content in coffee as influenced by grinding and brewing techniques. Food Research International, 29(8), 785-789. Bell, L.N., 2000. Caffeine and theobromine contents of ready-to-eat chocolate cereals. Journal of the Academy of Nutrition and Dietetics, 100(6), p.690.Brunetto, M.D.R., Gutierrez, L., Delgado, Y., Gallignani, M., Zambrano, A., Gomez, A., Ramos, G. and Romero, C., 2007. Determination of theobromine, theophylline and caffeine in cocoa samples by a high-performance liquid chromatographic method with on-line sample cleanup in a switching-column system. Food Chemistry, 100(2), 459-467.Carloni, P., Tiano, L., Padella, L., Bacchetti, T., Customu, C., Kay, A. and Damiani, E., 2013. Antioxidant activity of white, green and black tea obtained from the same tea cultivar. Food Research International, 53(2), 900-908.Chan, E.W.C., Eng, S.Y., Tan, Y.P., Wong, Z.C., Lye, P.Y. and Tan, L.N., 2012. Antioxidant and sensory properties of hai herbal teas with emphasis on thunbergia laurifolialindl. Journal of Science of Chiang Mai , 39(4), 599-609.Chan, E.W.C., 2009. Bioactivities and chemical constituents of leaves of some Etlingera species (Zingiberaceae) in Peninsular Malaysia. Thesis (Ph.D). Monash University Sunway Campus Malaysia.Chan, E.W.C., Lim, Y.Y. and Mohammed, O., 2007. Antioxidant and antibacterial activity of leaves of Etlingera species (Zingiberaceae) in Peninsular Malaysia. Food Chemistry, 104, 1586-1593. Chan, E.W.C., Lim, Y.Y., Wong, L.F., Lianto, F.S., Wong, S.K., Lim, K.K., Joe, C.E. and Lim, T.Y., 2008. Antioxidant and tyrosinase inhibition properties of leaves and rhizomes of ginger species. Food Chemistry, 109, 477-483. Chan, E.W.C., Lim, Y.Y., Wong, S.K., Lim, K.K., Tan, S.P., Lianto, F.S. and Yong, M.Y., 2009a. Effects of different drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chemistry, 113, 166-172.Chan, E.W.C., Lim, Y.Y., Ling, S.K., Tan, S.P., Lim, K.K. and Khoo, M.G.H., 2009b. Caffeoylquinic acids from leaves of Etlingera species (Zingiberaceae). LWT - Food Science and Technology, 42, 1026-1030. Chan, E.W.C., Lim, Y.Y., Chong, K.L., Tan, J.B.L. and Wong, S.K., 2010. Antioxidant properties of tropical and temperate herbal teas. Journal of Food Composition and Analysis, 23, 185-189. de Rezende Mudenuti, N.V., de Camargo, A.C., Shahidi, F., Madeira, T.B., Hirooka, E.Y. and Grossmann, M.V.E., 2018. Soluble and insoluble-bound fractions of phenolics and alkaloids and their antioxidant activities in raw and traditional chocolate: A comparative study. Journal of Functional Foods, 50, 164-171. del Rosario Brunetto, M., Gutiérrez, L., Delgado, Y., Gallignani, M., Zambrano, A., Gomez, A., Ramos, G. and Romero, C., 2007. Determination of theobromine, theophylline and caffeine in cocoa samples by a high-performance liquid chromatographic method with on-line sample cleanup in a switching-column system. Food Chemistry, 100(2), 459-467. Duan, Y., Song, X., Yang, Y., Li, L., Zhao, Y., Wang, Y., Fang, W. and Zhu X., 2018.  Correlation between Sensory Evaluation Scores and Flavor Attributes of Black Teas from Around the World. Journal of Food Processing and Technology, 9(4), 1-9. Franco, R., Oñatibia-Astibia, A. and Martínez-Pinilla, E., 2013. Health benefits of methylxanthines in cacao and chocolate. Nutrients, 5(10), 4159-4173. Krysiak, W., 2011. Effects of convective and microwave roasting on the physicochemical properties of cocoa beans and cocoa butter extracted from this material. Grasas Y Aceites, 62(4), 467-478. Lawless, H. and Heymann, H., 2013. Sensory evaluation of food: principles and practices. New  York: Springer Science and Business Media, 1-6. Lee, C., 2014. Planting Cocoa – Challenges And Reality In Malaysia [Online].Available from:http://www.iipm.com.my/ipicex2014/docs/2012/oral/PLANTING%20COCOA-%20CHALLENGES%20AND%20REALITY%20IN%20MALAYSIA.pdf [Accessed 10 May 2018]. Lee, J.,2009. Green Tea: Flavour characteristics of a wide range of teas including brewing, processing, and storage variations and consumer acceptance of teas in three countries. Thesis (PhD). Kansas State University. Lee, J. and Chambers, D.H., 2009. Sensory descriptive evaluation: brewing methods affect flavour of green tea. Asian Journal of Food and Agro-Industry, 2(4), 427-439. Lee, J. and Chambers, D.H., 2007. A lexicon for flavour descriptive analysis of green tea. Journal of Sensory Studies, 22(3), 256-272. Lee,K.W., Kim,Y.J., Lee,H.J., and Lee,C.Y., 2003. Cocoa has more phenolic phytochemicals and a higher antioxidant capacity than teas and red wine. Journal of Agriculture and Food Chemistry, 51, 7292-7295. Lee, S.M., Chung, S.S., Lee, O.H., Lee, H.S., Kim, Y.K., and Kim, O.K., 2008. Development of Sample Preparation, Presentation Procedure and Sensory Descriptive Analysis of Green Tea. Journal of Sensory Studies, 23, 450-467. Lee, S.M., Lee, H.S., Kim, K.H. and Kim, K.O., 2009. Sensory Chracteristics and Consumer Acceptability of Decaffeinated Green Teas. Journal of Food Science, 74(3), 135-141. Lim, Y.Y., Lim, T.T. and Tee, J.J., 2007. Antioxidant properties of several tropical fruits: A comparative study. Food Chemistry, 103(3), 1003-1008.Meilgaard, M., Civille, G.V. and Carr B.T., 1999. Sensory evaluation techniques, 3rd ed. Boca Raton: CRC Press, 59-251.Nagai, T., Inoue, R., Inoue, H. and Suzuki, N., 2003. Preparation and antioxidant properties of water extract of propolis. Food chemistry, 80(1), 29-33.Nawrot, P., Jordan, S., Eastwood, J., Rotstein, J., Hugenholtz, A. and Feeley, M., 2003. Effects of caffeine on human health. Food Additives and Contaminants, 20(1), 1-30.Paixão, N., Perestrelo, R., Marques, J.C. and Câmara, J.S., 2007. Relationship between antioxidant capacity and total phenolic content of red, rosé and white wines. Food Chemistry, 105(1), 204-214. Ramli, N., Hassan, O., Said, M., Samsudin, W. and Idris, N.A., 2006. Influence of roasting conditions on volatile flavour of roasted Malaysian cocoa beans. Journal of Food Processing and Preservation, 30(3), 280-298. Rocha, I.S., de Santana, L.R.R., Soares, S.E. and Bispo, E.D.S., 2017. Effect of the roasting temperature and time of cocoa beans on the sensory characteristics and acceptability of chocolate. Food Science and Technology (Campinas), 37(4), 522-530. Sanchez, J.M., 2017. Methylxanthine content in commonly consumed foods in spain and determination of its intake during consumption. Journal of Food Science & Nutrition, 6(12), 109. Stark, T., Bareuther, S. and Hofmann, T., 2006. Molecular definition of the taste of roasted cocoa nibs (Theobroma cacao) by means of quantitative studies and sensory experiments. Journal of Agricultural And Food Chemistry, 54(15), 5530-5539.
 
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